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14 octobre 2020

Paralepistopsis gen. nov. and Paralepista by A. Vizzini & E. Ercole

ISSN (print) 0093-4666                                                                         © 2012. Mycotaxon, Ltd.                                               ISSN (online) 2154-8889


MYCOTAXON

http://dx.doi.org/10.5248/120.253

Volume 120, pp. 253–267 April–June 2012


 

Paralepistopsis gen. nov. and Paralepista (Basidiomycota, Agaricales)

Alfredo Vizzini* & Enrico Ercole

Dipartimento di Scienze della Vita e Biologia dei Sistemi - Università degli Studi di Torino,Viale Mattioli 25, I-10125, Torino, Italy
*Correspondence to: alfredo.vizzini@unito.it

 

AbstractParalepistopsis, a new genus in Agaricales, is proposed for the rare toxic species, Clitocybe acromelalga Ichimura (1918) from Asia (Japan and South Korea), and Clitocybe amoenolens from North Africa (Morocco) and southern and southwestern Europe and C. acromelalga from Asia (Japan and South Korea).

Paralepistopsis is distinguished from its allied clitocyboid genera by a Lepista flaccida-like habit, a pileipellis with diverticulate hyphae, small non-lacrymoid basidiospores with a smooth slightly cyanophilous and inamyloid wall, and the presence of toxic acromelic acids.

Combined ITS-LSU sequence analyses place Paralepistopsis close to Cleistocybe and Catathelasma within the tricholomatoid clade. Our phylogenetic analysis further supports Lepista subg. Paralepista (= Lepista sect. Gilva) as an independent clitocyboid evolutionary line.

We recognize the genus Paralepista, for which we propose twelve new combinations.

Key wordsAgaricomycetes, erythromelalgia / acromelalgic syndrome, Clitocybe sect. Gilvaoideae, / Catathelasma clade.

 

Introduction

     The genus Clitocybe (Fr.) Staude traditionally encompassed saprobic agarics that produce fleshy basidiomata with often adnate-decurrent lamellae, convex to funnel-shaped pilei, usually a whitish to pinkish yellow spore print, and smooth non-amyloid basidiospores (Kühner 1980, Singer 1986, Bas 1990, Raithelhuber 1995, 2004).

     Recent molecular studies that included a significant number of Clitocybe species (Moncalvo et. al. 2002, Redhead et al. 2002, Matheny et al. 2006, Vizzini et al. 2010 a,b, 2011) have shown that taxa in this traditional genus do not form a monophyletic group but rather a heterogeneous artificial set of disparate and (in many cases) phylogenetically unrelated taxa (the so-called clitocyboid fungi or Clitocybe s.l.).

     Clitocybe amoenolens is a rare and rather localized species known thus far only from Morocco, southern France, northern and


 254 ... Vizzini & Ercole

 central Spain, and central Italy (Malençon & Bertault 1975, Bon 1987, Poumarat & Neville 1993, Contu et al. 1999, Moreau et al. 2001, Martínez et al. 2010).

     It was responsible, first in France (Fourré 1997, Charignon & Garcin 1998, Moreau et al. 2001, Saviuc et al. 2001, 2002) and then in Italy (Leonardi et al. 2002, Marinetti & Recchia 2005), for induced erythromelalgia (= acromelalgic syndrome sensu Saviuc et al. 2001), a poisoning syndrome caused by the ingestion of C. acromelalga in Japan (Nakamura et al. 1987).

     This syndrome is characterized by varying degrees of tingling sensations, followed by intense burning pain in the extremities but predominantly in the feet (Saviuc & Danel 2006). Clitocybe amoenolens was confused with edible mushrooms in the Lepista flaccida complex (e.g., L. flaccida (Sowerby) Pat., L. lentiginosa (Fr.) Bresinsky, L. gilva (Pers.) Pat.) and with Infundibulicybe gibba (Pers.) Harmaja (Fourré 1997, Moreau et al. 2001).

     Clitocybe amoenolens shows features intermediate between Clitocybe s.s. (smooth spores) and Lepista subg. Paralepista (cream spore-print, spotted pileus, lamellae separable from context, and cyanophilic spores released in tetrads), making its generic position uncertain.

    Using recent French and Italian collections of C. amoenolens, we investigated its phylogenetic position within the clitocyboid fungi through morphological and molecular analyses and expanded its known geographic distribution.

Materials & methods

Morphology

     Macromorphological features were described from fresh specimens. Microscopical preparations from dried material were rehydrated in 3% KOH and stained in Congo red, Cresyl Blue, Cotton Blue and Melzer’s reagent.

     Basidiospore measurements are based on means of 120 spores from prints (four collections), stained in Melzer’s reagent. The basidia width was measured at the widest part and the length from the apex (sterigmata excluded) to basal septum. The following abbreviations are used in text: L = number of entire lamellae; l = number of lamellulae between each pair of entire lamellae; Q = the quotient of length and width of the spores in side view; Qm = average quotient. Colour terms in capital letters (e.g. Pale Ochraceous-Buff) are those of Ridgway (1912). Herbarium acronyms follow Thiers (2011).

     Author citations follow Index Fungorum (http://www.indexfungorum.org/authorsoffungalnames.htm). All examined collections are housed at TO. The new genus and new combinations are deposited in MycoBank (http://www.mycobank.org/DefaultPage.aspx ). DNA extraction, PCR amplification, and DNA sequencing Genomic DNA was isolated from 1 mg of herbarium material (Table 1) using the DNeasy Plant Mini Kit (Qiagen, Milan Italy) according to the manufacturer’s instructions. Universal primers ITS1F/ITS4 were used for the ITS region amplification (White et al. 1990, Gardes & Bruns 1993) and primers LR0R/LR7 for the LSU rDNA amplification (Vilgalys & Hester 1990, Vilgalys lab unpubl., http://www.botany.duke.edu/fungi/

DNA extraction, PCR amplification, and DNA sequencing

Genomic DNA was isolated from 1 mg of herbarium material (Table 1) using the DNeasy Plant Mini Kit (Qiagen, Milan Italy) according to the manufacturer’s instructions. Universal primers ITS1F/ITS4 were used for the ITS region amplification (White et al. 1990, Gardes & Bruns 1993) and primers LR0R/LR7 for the LSU rDNA amplification (Vilgalys & Hester 1990, Vilgalys lab unpubl., http://www.botany.duke.edu/fungi/mycolab).


Paralepistopsis gen, nov. and Paralepista... 255

Table 1. Collections newly sequenced in this study.

Species                              GenBank acc. numbers             Source, country,  
                                             
ITS               LSU                   date, collector

Paralepistopsis amoenolens 1   JQ585653           JQ585654            TO AV2004, FRANCE,
                                                                                                         
02/09/2011, G. Moretto

Paralepistopsis amoenolens JQ585655                  —                        TO AV2007, ITALY,  
                                                                                                         12/11/2011, S. Anselmin
Paralepista flaccida 1              JQ585656              JQ585657                 TO AV2008, ITALY,
                                                                                                           02/09/2011, G. Moretto
 
Paralepista flaccida 2             JQ585658                JQ585659                TO AV2009, ITALY,
                                                                                                            20/10/2011, A. Vizzini
Paralepista gilva                    JQ585660                JQ585661                 TO AV2010, ITALY,
                                                                                                             09/11/2011, A. Vizzini
 

 256      ... Vizzini & Ercole
Amplification reactions were performed in a PE9700 thermal cycler (Perkin-Elmer, Applied Biosystems) following Vizzini et al. (2010 b). PCR products were purified with the AMPure XP kit (Beckman) and sequenced by DiNAMYCODE srl (Turin, Italy) and MACROGEN Inc. (Seoul, Republic of Korea). Sequences were assembled and edited with the phred/phrap/consed software suite. The sequences were submitted to GenBank and their accession numbers are reported in Table 1 and Figure 1.
    
Sequence alignment and phylogenetic analysis
The sequences obtained in this study were checked and assembled using Geneious v5.3 (Drummond et al. 2010) and compared to those available in GenBank (http://www.ncbi.nlm.nih.gov/Genbank/) using the blastn algorithm. Based on the blastn results, sequences were selected according to the outcomes of recent phylogenetic studies on Agaricales (Matheny et al. 2006, Binder et al. 2010, Vizzini et al. 2011).
A combined ITS and LSU sequence analysis was carried out using sequences from the same strain or specimen.
Xeromphalina campanella (GU320006 and GU320009) was used as outgroup. Alignments were generated using MAFFT (Katoh et al. 2002) with default conditions for gap openings and gap extension penalties.
The sequence alignment, its manual adjustment, and the best-fit models estimation follow Vizzini et al. (2010b). The GTR+G and GTR+G substitution models were used in the ITS and LSU analyses, respectively.
A partitioned matrix was used in all the analyses. Molecular-phylogenetic analyses were performed using the Bayesian Inference (BI) and Maximum Likelihood (ML) approaches.
BI using Monte Carlo Markov Chains (MCMC) was carried out with MrBayes 3.1.2 (Huelsenbeck and Ronquist 2001).
Four incrementally heated simultaneous MCMC were run over 5.000.000 generations, under model assumption. Trees were sampled every 500 generations resulting in an overall sampling of 10.001 trees. The “burn-in” value was evaluated using Tracer 1.5 (Rambaut & Drummond 2007). The first 20% of trees was discarded as “burn-in”.
For the remaining trees, a majority rule consensus tree showing all compatible partitions was computed to obtain estimates for Bayesian Posterior Probabilities (BPP).
ML estimation was performed through RAxML v.7.0.4 (Stamatakis 2006) with 1.000 bootstrap replicates (Felsenstein 1985) using the

 256 ... Vizzini & Ercole

Paralepistopsis-page-004 fig 1Figure 1. Tricholomatoid clade. Bayesian phylogram obtained from the combined ITS-LSU sequence alignment. Support values for clades that are supported in either the Bayesian (Posterior Probabilities values – BPP) or Maximum likelihood (ML Bootstrap percentage – MLB) analyses are indicated. BPP > 0.70 and MLB > 50% are given above branches. Numbers (1, 2) refer to the Paralepistopsis and Paralepista collections reported in Table 1.

GTRGAMMA algorithm for both ITS and LSU to perform a tree inference and search for a good topology. Support values from bootstrapping runs (MLB) were mapped on the globally best tree using the “-f a” option of RAxML and “-x 12345” as a random seed to invoke the novel rapid bootstrapping algorithm. Only BPP values over 0.70 and MLB over 50% are reported in the resulting tree (Fig. 1). Pairwise % identity values of ITS sequences were calculated using MEGA 5.0 (Tamura et al. 2011).

Results

Molecular results

The combined data set comprises a total of 71 taxa (including 66 from GenBank) and is 2412 base pairs long. The ITS and LSU datasets are 796 and 1616 base pairs long, respectively. Topologies of the combined ITS and LSU Bayesian and Maximum Likelihood trees are congruent (Fig. 1). In both analyses the two Clitocybe amoenolens collections (ITS pairwise identity value = 99.9%) clearly cluster with the C. acromelalga collection (BPP 1 and MLB 100%) in the / Catathelasma clade, a monophyletic group


 Paralepistopsis gen, nov. and Paralepista... 257

in the tricholomatoid clade (Matheny et al. 2006, Ammirati et al. 2007). The ITS pairwise identity value between the C. acromelalga and C. amoenolens sequences is 94.4%; accepting an intraspecific variability lower than 3% (Nilsson et al. 2008), C. amoenolens and C. acromelalga should be considered distinct species. Cleistocybe vernalis Ammirati et al. cluster sister to the C. acromelalga / C. amoenolens pair (BPP 0.82);

Catathelasma Lovejoy is basal to this group with low BPP and MLB values. Clitocybe amoenolens and Clitocybe acromelalga have no phylogenetic relationship with other Clitocybe species. Accordingly, we propose to establish for them a new genus, Paralepistopsis. Lepista flaccida and L. gilva are not phylogenetically related to the other Lepista species.

Taxonomy

Paralepistopsis Vizzini, gen. nov.

MycoBank MB564340A

Paralepista differt sporis levis, haud subglobosis, atque praesentia acidi acromelalgici qua de causa venenatae species sunt. et in stuctura molecularis (ITS-spatiis internis transcriptis et LSU DNA).

Type species Clitocybe amoenolens Malençon

Etymologynamed in reference to its resemblance to Paralepista species.

Basidiomata agaricoid (with distinct pileus, lamellae and stipe), resembling those of Paralepista gilva, veils absent, spore-print whitish to cream, basidiospores thin-walled, smooth, inamyloid and slightly cyanophilous, pileal surface a cutis of repent to interwoven, cylindrical hyphae, clamp-connections present, no sarcodimitic texture in any part of the basidioma. On the ground, never on wood.

Paralepistopsis amoenolens (Malençon) Vizzini, comb. nov. Fig. 2
MycoBank MB564341

Clitocybe amoenolens Malençon, in Malençon & Bertault, Flore des champignons superieurs du Maroc 2 - Trav. Inst. Sci. Chérifien, Sér. Bot. Biol. Vég. 33: 141 (1975).

Selected descriptions Malençon & Bertault (1975: 138–141); Moreau et al. (2001: 99–100, 101–103).

Selected iconography Malençon & Bertault (1975: pl. 8); Poumarat & Neville (1993: 48); Martínez et al. (2010: fig. 2, p. 104).

Pileus (2–)3.5–7(–8) cm diam., fleshy, sub-elastic, hemispherical to convex at first, becoming plano-convex and applanate, finally plano-concave, at times broadly umbonate; margin narrowly inrolled and decurved at first, remaining inrolled for a long time, then expanding to become somewhat wavy, shortly sulcate-striate or corrugated; not hygrophanous, occasionally appearing


 258 ... Vizzini & Ercol

hygrophanous when water soaked; surface slightly viscid when moist, at first entirely whitely pruinose, then pubescent-pruinose only at margin, typically diffracted-scaly near the disc, sometimes corrugated, wrinkled and areolate, later subglabrous often with more or less concentrically arranged watery, drop-like spots, especially near margin (as many Lepista species); at first cream-beige coloured (Capucine Buff, Pale Ochraceous-Salmon, Pale Ochraceous-Buff), then pinkish beige to rusty orange (Ochraceous-Buff, Zinc Orange) towards the centre.

Lamellae crowded to close (L = 40–48(–50), l = (0–)1–2(–3)), thick, interspersed with lamellulae, (2–)3–4(–5) mm broad, decurrent, easily separable from the pileus context, at times intervenose or forked towards the stipe, at first whitish then yellowish ochre (Ivory Yellow, Chamois) to pinkish beige (Pale Ochraceous-Salmon, Pale Ochraceous-Buff); edges even, entire, concolorous.

Stipe (2.5–)3–3.5(–5) cm long, 0.7–1.3 cm thick, short, central or subexcentric, equal or with a somewhat enlarged base, straight or recurved, concolorous with the pileus or slightly paler, minutely white pruinose at apex, glabrous elsewhere, stuffed with white medulla, becoming hollow; the base often with copious whitish tomentum with adhering Pinaceae needles and woody debris.

Context 4–11(–14) mm thick at disc, elastic, white in the pileus, whitish cream (Capucine Buff) in the stipe cortex, unchanging; taste mild, fungoid, subfarinaceous, slightly bitter-farinaceous after long mastication; odour strong, aromatic, floral, reminiscent of Inocybe corydalina, Tricholoma caligatum, Lepista irina, or Entoloma ameides.

Spore print whitish to pale cream (Light Buff, Pale Pinkish Buff).

Basidiospores (3.8–)4.0–5.4(–5.6) × (2.3–)3.2–4.0(–4.3) μm (n = 120), on average 4.8 × 3.4 μm, Q = 1.3–1.7, Qm = 1.42, broadly ellipsoid, hyaline, thin-walled, smooth, slightly cyanophilous, inamyloid, non-dextrinoid, usually with only a single oil drop and a distinct truncated apiculus up to 0.7 μm long, mostly adhering together in tetrads in dried specimens.

Basidia (25–)30–37(–38) × 5–6(–7) μm, cylindro-clavate, usually four-spored, occasionally two-spored, sterigmata up to 5 μm long. Hymenophoral trama regular in young stages, but subirregular in mature basidiomata, consisting of hyaline, elongated, cylindrical hyphae 4–6(–8) μm broad.

Pleurocystidia absent. Lamella edges fertile, with rare, scattered cells, not well differentiated from basidia, 15–45(–60) × 2.5–5(–7) μm, cylindric to subfusiform or sublageniform, often curved and flexuous, sometimes forked at apex, hyaline, thin-walled.

Pileipellis duplex: upper layer (suprapellis) a soon disappearing, slightly gelatinous thin cutis (10–30 μm thick), of cylindrical hyphae, 1–3 μm broad; lower layer (subpellis) (150–350 μm thick) composed of densely arranged parallel to slightly interwoven hyphae (4–6 μm in diam.), terminal elements scattered, erect and repent cylindrical to fusiform-lageniform, sometimes with short lateral outgrowths. Pileitrama consisting of cylindrical or slightly


260 ... Vizzini & Ercole

inflated, smooth hyphae, (4–)5–9(–12) μm broad, irregularly arranged, and with aeriferous lacunae.

Stipitipellis a cutis consisting of hyphae 3–4 μm broad; towards the stipe apex with numerous thin-walled, flexuous, cylindrical to subfusiform caulocystidioid elements, 20–35 × 3–6 μm. Stipititrama formed by hyphae 6–7 μm broad. Thromboplerous hyphae (= oleiferous hyphae sensu Clémençon 2004) present especially in subpellis, pileus and hymenophoral trama. Pigment (yellowish) usually parietal and intracellular (cytoplasmatic) in the pileipellis; sometimes minutely incrusting or forming extracellular masses and granules. Clamp connections present at nearly all septa.

Habitat & distribution: scattered, gregarious, occasionally subcaespitose on pinaceous needle-beds and debris, often together with Lepista flaccida, on calcareous soil.

Autumn. Very rare; known only from Morocco, France, Spain, and Italy.

Material studied: FRANCE, Savoie, Haute-Maurienne valley, Lanslebourg-Mont-Cenis, 02 Sept 2011, litter of Picea abies,1400 m asl, leg. G. Moretto (TO AV2004); 14 Oct 2011, leg. G. Moretto (TO AV2005).ITALY, Piedmont, High Susa Valley, Salbertrand, Parco Naturale del Gran Bosco di Salbertrand, 20 Oct 2011, litter of Picea abies, 1500 m asl, leg. A. Vizzini (TO AV2006); Sauze d’Oulx, Parco Naturale del Gran Bosco di Salbertrand, 12 Nov 2011, litter of Larix decidua, 1550 m asl, leg. S. Anselmino (TO AV2007).

Clitocybe amoenolens was originally described from Morocco, in the Middle Atlas, growing among Cedrus atlantica litter in a high-altitude cedar forest (1600–1700 m a.s.l.) mixed with Ilex aquifolium and Quercus ilex, on calcareous soil (Malençon & Bertault 1975).

It has recently been found in southern France (Bon 1987, Poumarat & Neville 1993) and in the Maurienne Valley (Charignon & Garcin 1998, Fourré 1997) in coniferous forests (Pinus sylvestris, Larix decidua, Picea abies) and always on calcareous soil.

Clitocybe amoenolens has been responsible for several acromelalgic-type poisonings in the Maurienne Valley (Savoie, France) (Moreau et al. 2001, Saviuc & Danel 2006). It also has been found in the Abruzzi region (Centre Italy) under Pinus nigra (Contu et al. 1999), P. nigra, and Cedrus spp. (Leonardi et al. 2002) and P. nigra and Larix decidua (Leonardi & Maggi 2007), and poisoning cases referable to C. amoenolens have been recognised in this region (Leonardi et al. 2002, Marinetti & Recchia 2005).

Finally, the species has been reported from Spain (Martínez et al. 2010), where it was collected in the autonomous regions of La Rioja (Picea abies) and Castilla-La Mancha (Pinus pinaster and Cupressus arizonica or P. nigra and Quercus petraea), close to the north and centre of Spain, respectively. Our collections are the first record from northern Italy. According to our observations and bibliographic data, this species seems strictly restricted to Pinaceae in higher altitude thermophilic forests on calcareous soils.


  Paralepistopsis gen, nov. and Paralepista... 261

Paralepistopsis acromelalga (Ichimura)Vizzini, comb. nov., MycoBank MB564342

Clitocybe acromelalga Ichimura, Bot. Gaz. (Tokyo) 65: 110 (1918).

Selected descriptions Ichimura (1918: 110); Moreau et al. (2001: 109–111).

Selected iconography Guez (2010: (http://www.mycodb.fr/fiche.php?genre=Clitocybe&espece=acromelalga ).

Habitat & distribution: Clitocybe acromelalga, described from Japan (Ichimura 1918, Imazeki & Hongo 1957, Imazeki et al. 1988; Romagnesi 1989, Guez 1990), also occurs in South Korea (Lee & Hong 1985). It was reported as growing on both angiosperm (Phyllostachys bambusoides, Acer palmatum, Zelkova serrata) and gymnosperm (Cryptomeria japonica) litter.

Phylogeny and specific delimitation

In our combined ITS-LSU phylogenetic tree (Fig. 1) Clitocybe acromelalga and its sister C. amoenolens are not closely related to C. nebularis (Batsch) P. Kumm., the type of the genus Clitocybe (Redhead et al. 2002), nor to other Clitocybe species or allied taxa. As these two species represent a new phyletic line of clitocyboid fungi, it seems most appropriate to transfer them to the new genus Paralepistopsis.

Based on its habit, coloured lamellae and small spores, Bon (1997)* and Moreau et al. (2001) placed C. amoenolens traditionally in subg. Clitocybe sect. Gilvaoideae Harmaja, where it occupies an isolated position. Contu et al. (1999), focusing on hymenial features (basidia longer than 30 μm), placed it in subgen. Hygroclitocybe Bon sect. Clavipedes Harmaja, a subgenus shown in recent molecular analyses (Redhead et al. 2002, Vizzini et al. 2011) to be artificial and heterogeneous.

The traditionally defined Lepista (Fr.) W.G. Sm. —clitocyboid fungi with a pinkish yellow spore print, usually separable lamellae, and inamyloid cyanophilous ornamented [verruculose to spiny] basidiospores (Singer 1986, Bon 1997, Consiglio & Contu 2003)— is a polyphyletic genus (Fig. 1).

The species of Lepista subg. Paralepista (= Lepista sect. Gilva), which combine very crowded decurrent lamellae with subglobose to largely ellipsoidal spores, are not closely related either to Lepista s.s. or to other taxa in the tricholomatoid clade.

Consequently we accept this lineage as a distinct genus and propose Paralepista for Lepista flaccida, L. gilva and allies.

Following Bigelow (1985), Bon (1991), and Raithelhuber (2004), we list below all the taxa accepted in Paralepista :

Paralepista Raithelh., Gattung Clitocybe 1: 17 (1981).

Type speciesAgaricus inversus Scop.

Lepista subg. Paralepista (Raitelh.) Bon, Doc. Mycol. 26(102): 18 (1996).

= Clitocybe sect. Eulepistae Singer, Ann. Mycol. 41: 40 (1943).


 

262 ... Vizzini & Ercole
(1985), Bon (1991), and Raithelhuber (2004), we list below all the taxa accepted in Paralepista :
  • Paralepista Raithelh., Gattung Clitocybe 1: 17 (1981).
    = Lepista sect. Gilva Harmaja, Karstenia 18: 53 (1978). Lepista sect. Eulepista ” Konrad 6 & Maubl., Icon. Select. Fung. 6(10): 350 (1936), nom. inval.Lepista sect. Inversae ” Singer & Clémençon, Nova Hedwigia 23: 310 (1973 [“1972”]), nom. inval.
  • Paralepista abdita (Dörfelt)Vizzini,comb. nov., MycoBank MB564343
    Lepista abdita Dörfelt, Boletus 1(2): 37 (1997).
  • Paralepista ameliae (Arcang.)Vizzini, comb. nov., MycoBank MB 564344
    Clitocybe spinulosa var. ameliae Arcang., Nuovo Giorn. Bot. Ital. 21: 434 (1889).
  • Paralepista biformis (Peck)Vizzini, comb. nov. , MycoBank MB 564345
    Clitocybe biformis Peck, Bull. N.Y. St. Mus. 150: 25 (1911).
  • Paralepista concentrica Raithelh., Metrodiana 23: 122 (1996).
  • Paralepista femoralis (H.E. Bigelow) Vizzini, comb. nov., MycoBank MB 564346
    Clitocybe femoralis H.E. Bigelow, Sydowia 36: 14 (1983).
  • Paralepista flaccida (Sowerby) Vizzini, comb. nov., MycoBank MB 564347
    Agaricus flaccidus Sowerby, Col. Fig. Engl. Fungi 2: pl. 185 (1799).
  • Paralepista flaccida var. fibrillosa (Malençon) Vizzini, comb. nov., MycoBank MB 564348
    Clitocybe flaccida var. fibrillosa Malençon, in Malençon & Bertault, Flore des champignons superieurs du Maroc 2 - Trav. Inst. Sci. Chérifien, Sér. Bot. Biol. Vég. 33: 157 (1975).
  • Paralepista gilva (Pers.) Vizzini, comb. nov., MycoBank MB 564349
    Agaricus gilvus Pers., Syn. Meth. Fung.: 448 (1801).Paralepista gilva ” Raithelh., Metrodiana 23: 117 1996),  nom. inval.
  • Paralepista inversa (Scop.) Raithelh., Gattung Clitocybe 1: 17 (1981).
    Agaricus inversus Scop., Fl. Carniol., Ed. 2, 2: 445 (1772).
  • Paralepista lentiginosa (Fr.) Vizzini, comb. nov., MycoBank MB 564350
    Agaricus lentiginosus Fr., Epicr. Syst. Mycol.: 69 (1838).
  • Paralepista maculosa (Sacc.) Vizzini, comb. nov., MycoBank MB 564352
    Agaricus maculosus Peck, Bull. Buffalo Soc. Nat. Sci. 1: 45 (1873), nom. illegit., non Pers. (1801).
    Clitocybe maculosa Sacc., Syll. Fung. 5: 183 (1887).

Paralepistopsis gen, nov. and Paralepista... 263

  • Paralepista pseudoparilis (Enderle & Contu) Vizzini, comb. nov. MycoBank MB 564353
    Lepista pseudoparilis Enderle & Contu, Beitr. Kenn. Pilze Mittel. 13: 12 (2000).
  • Paralepista repanda (Raithelh.) Raithelh., Metrodiana 23: 121 (1996).
    Lepista repanda Raithelh., Metrodiana 14: 21 (1986 [“1985”]).

 

  • Paralepista shafferi (H.E. Bigelow) Vizzini, comb. nov., MycoBank MB 564354
    Clitocybe shafferi H.E. Bigelow, Beih. Nova Hedwigia 81: 339 (1985).
  • Paralepista splendens (Pers.) Vizzini, comb. nov., MycoBank MB 564355
    Agaricus splendens Pers., Syn. Meth. Fung.: 452 (1801).

 

Discussion

Paralepistopsis species are characterized by a habit (decurrent and crowded lamellae) and colours (ochre-orange tinges) reminiscent of Paralepista or Infundibulicybe Harmaja, a whitish to cream spore print, smooth cyanophilic spores often arranged in tetrads in dried specimens and rarely exceeding 5(–6) μm in length.

Paralepista differs in having strongly ornamented spores (Raithelhuber 1995, 2004); Infundibulicybe is distinguishedby smooth lacrymoid spores with confluent bases and cyanophobic spore walls (Harmaja 2003).

Paralepistopsis clusters with Cleistocybe Amirati et al. and Catathelasma in the / catathelasma clade. Because of the low resolution and lack of BPP and MLB support within the tree, a more precise, accurate position for the new genus could not be suggested. The presence of decurrent lamellae, confluent pileus and stipe, pale reddish brown colouration, and growth on soil are characters shared by Paralepistopsis, Cleistocybe, and Catathelasma.

Cleistocybe and Catathelasma are distinguished from Paralepistopsis mainly by a partial veil, divergent to interwoven hymenophoral trama, and larger cyanophobic spores (Ammirati et al. 2007, Vizzini 2009); in addition Catathelasma spores are amyloid (Singer 1986). Paralepistopsis amoenolens is delimited by a unique combination of macro-/micromorphological and chemical features, such as i) a strong aromatic, floral odour reminiscent of Tricholoma caligatum, Inocybe corydalina, Lepista irina, and Entoloma ameides caused by volatile metabolites identified by Fons et al. (2006) as methyl-(E)-cinnamate (also a key odorant of T. caligatum), methyl-benzoate, (E)-nerolidol, and methylanthranilate; ii) lamellae easily separating from the pileus context; iii) a cream coloured spore print; iv) smooth cyanophilic spores often arranged in tetrads; v) basidia reaching 35–40 μm; vi) pileipellis hyphae with short diverticula; vii) abundant thromboplerous hyphae; viii) and the presence of the toxic metabolite, acromelic acid A, a powerful neurotoxic


 264 ... Vizzini & Ercole

amino acid responsible for erythromelalgic poisoning and structurally homologous with kainic acid (a strong agonist of non-N-methyl-D-aspartate glutamate receptor subtypes) and domoic acid (Bessard et al. 2004).

Paralepistopsis acromelalga differs from P. amoenolens morphologically in a darker pileus and stipe, a pileus that soon becomes depressed, more crowded lamellae, a different odour, thromboplerous hyphae occurring only rarely, smaller spores (Ichimura 1918, Romagnesi 1989, Guez 1990, Miyauchi 1998, Moreau et al. 2001), and a more complex metabolite pattern (presence of acromelic acids A–E with 19 other toxins; Konno et al. 1983, 1988; Fushiya et al. 1990, 1992; Saviuc & Danel 2006). Additionally, our analyses show only a 91% pairwise ITS sequence identity between P. acromelalga and P. amoenolens.

Singer (1986) transferred P. acromelalga to the heterogeneous genus Neoclitocybe Singer based on the presence of rare diverticulate hyphae in the pileipellis. Based on their small spores and the Paralepista-like habit, C.gilvaoides Kauffman and C. gracilis (H.E. Bigelow & A.H. Sm.) Harmaja (sect. Gilvaoideae) from the coniferous forests of North America and Scandinavia (Bigelow 1985, Harmaja 1969) may also belong to Paralepistopsis, but more recently collected specimens are needed to perform molecular and biochemical analyses.

Acknowledgements

We would like to thank Vladimír Antonín (Moravian Museum, Brno, Czech Republic), Joe Ammirati (University of Washington, Seattle, USA), and Shaun Pennycook (Auckland, New Zealand) for their pre-submission reviews. Our most sincere thanks are due to Marco Contu (Olbia, Italy) for helpful suggestions and comments.

Literature cited

Ammirati JF, Parker AD, Matheny PB. 2007. Cleistocybe, a new genus of Agaricales. Mycoscience 48: 282–289. http://dx.doi.org/10.1007/s10267-007-0365-5

Bas C. 1990. Tricholomataceae R. Heim ex Pouz. 65–70, in: C Bas et al. (eds). Flora Agaricina Neerlandica 2. A.A. Balkema, Rotterdam.

Bessard J, Saviuc P, Chane-Yene Y, Monnet S, Bessard G. 2004. Mass spectrometric determination of acromelic acid A from a new poisonous mushroom: Clitocybe amoenolens. J. Chromatogr. A. 1055: 99–107. http://dx.doi.org/10.1016/j.chroma.2004.08.133

Bigelow HE. 1985. North American species of Clitocybe. Part II. Beih. Nova Hedwigia 81: 281–471.

Binder M, Larsson K.-H, Matheny PB, Hibbett DS. 2010. Amylocorticiales ord. nov. and Jaapiales, ord. nov.: early diverging clades of Agaricomycetidae were dominated by corticioid forms. Mycologia 102: 865–880. http://dx.doi.org/10.3852/09-288

Bon M. 1987. Quelques espèces intéressantes étudiées au stage FMDS de Saint Germain Monts d’Or. Bull. Féd. Mycol. Dauphiné-Savoie 105: 28–30.

Bon M. 1997. Les clitocybes, omphales et ressemblants. Doc. Mycol., mémoires hors-série 4: l–174.

Charignon Y, Garcin R. 1998. Un nouveau champignon toxique en France. Bull. Féd. Mycol. Dauphiné-Savoie 149: 11–14.


 Paralepistopsis gen, nov. and Paralepista... 265

Clémençon H. 2004. Cytology and plectology of the Hymenomycetes. Bibl. Mycol. 199: 1–488.

Consiglio G, Contu M. 2003. Il genere Lepista in Italia. Riv. Micol. 46: 131–176.

Contu M, Signorello P, Anastase A. 1999. Clitocybe amoenolens Mal. in Abruzzo con osservazioni sulla sua posizione sistematica. Micol. Veget. Medit. 48: 16–18.

Drummond AJ, Ashton B, Buxton S, Cheung M, Cooper A, Duran C, Field M, Heled J, Kearse M, Markowitz S, Moir R, Stones-Havas S, Sturrock S, Thierer T, Wilson A. 2010. Geneious v5.3. Available from http://www.geneious.com/.

Felsenstein J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791. http://dx.doi.org/10.2307/2408678

Fons F, Rapior S, Fruchier A, Saviuc P, Bessiere J. 2006. Volatile composition of Clitocybe amoenolens, Tricholoma caligatum and Hebeloma radicosum. Cryptogam. Mycol. 27: 45–55.

Fourré G. 1997. Intoxications: un sosie du Lepista inversa a produit en Savoie un terrifiant syndrome...japonais. Bulletin de la Société Mycologique du Massif d’Argenson 16: 6–11.

Fushiya S, Sato S, Kazasawa T, Kusano G, Nozoe S. 1990. Acromelic acid C. A new toxic constituent of Clitocybe acromelalga: a efficient isolation of acromelic acids. Tetrahedron Lett. 31: 3901–3904. http://dx.doi.org/10.1016/S0040-4039(00)97501-4

Fushiya S, Sato S, Kera Y, Nozoe S. 1992. Isolation of acromelic acids D and E from Clitocybe acromelalga. Heterocycles 34: 1277–1280. http://dx.doi.org/10.3987/COM-92-6065

Gardes M, Bruns TD. 1993. ITS primers with enhanced specificity for basidiomycetes – application to the identification of mycorrhizae and rusts. Mol. Ecol. 2: 113–118. http://dx.doi.org/10.1111/j.1365-294X.1993.tb00005.x

Guez D. 1990. Aperçu sur la flore mycologique du Japon. Bull. Féd. Mycol. Dauphiné-Savoie 116: 12–16.

Harmaja H. 1969. The genus Clitocybe (Agaricales) in Fennoscandia. Karstenia 10: 5–168.

Harmaja H. 2003. Notes on Clitocybe s. lato (Agaricales). Ann. Bot. Fenn. 40: 213–218.

Huelsenbeck JP, Ronquist F. 2001. MrBayes: Bayesian inference of phylogeny. Bioinformatics 17: 754–755. http://dx.doi.org/10.1093/bioinformatics/17.8.754

Ichimura T. 1918. A new poisonous mushroom. Bot. Gaz. (Tokyo) 65: 109–111. http://dx.doi.org/10.1086/332195

Imazeki R, Hongo T. 1957. Coloured illustrations of mushrooms of Japan, vol. I. Hoikusha Publ., Osaka.

Imazeki R, Otani Y, Hongo T. 1988. Fungi of Japan. Yama-Kei publ., Tokyo.

Katoh K, Misawa K, Kuma K, Miyata T. 2002.MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucl. Acids Res. 30: 3059–3066. http://dx.doi.org/10.1093/nar/gkf436

Konno K, Shirahama H, Matsumoto T. 1983. Isolation and structure of acromelic acid A and B. New kainoids of Clitocybe acromelalga. Tetrahedron Lett. 24: 939–942. http://dx.doi.org/10.1016/S0040-4039(00)81571-3

Konno K, Hashimoto K, Ohfune Y, Shirahama H, Matsumoto T. 1988. Acromelic acids A and B. Potent neuroexcitatory amino acids isolated from Clitocybe acromelalga. J. Am. Chem. Soc. 110: 4807–4815. http://dx.doi.org/10.1021/ja00222a044

Kühner R. 1980. Les Hyménomycètes agaricoïdes. Bull. Soc. Linn. Lyon 49, num. spéc.:1–1027.

Lee JY, Hong SW. 1985. Illustrated flora and fauna of Korea. No. 28. Mushrooms. Ministry of Culture and Education, Korea.

Leonardi M, Maggi S. 2007. Nuova segnalazione per Clitocybe amoenolens in Abruzzo. Micol. Veget. Medit. 22: 80.

Leonardi M, Ciulli G, Pacioni G, Recchia G. 2002. Una intossicazione collettiva da Clitocybe amoenolens riconducibile alla sindrome acromelalgica. Micol. Veget. Medit. 17: 133–142.


266 ... Vizzini & Ercole

 

Malençon G, Bertault R. 1975. Flore des champignons supérieurs du Maroc, tome 2. Trav. Inst. Sci. Chérifien, Sér. Bot. Biol. Vég. 33: 1–540.

Marinetti V, Recchia G. 2005. Nuovi casi di sindrome acromelalgica in Abruzzo. Boll. Gr. micol. G. Bres. (n.s.) 48: 39–43.

Martínez F, Martínez R, Meléndez A, Pérez Del Amo CM. 2010. Clitocybe amoenolens. Primera cita para España. Bol. Soc. Micol. Madrid 34: 103–112.

Matheny PB, Curtis JC, Hofstetter V, Aime MC, Moncalvo JM, Ge ZW, Yang ZL, Slot JC, Ammirati JF, Baroni TJ, Bougher NL, Hughes KW, Lodge DJ, Kerrigan RW, Seidl MT, Aanen DK, DeNitis M, Daniele GM, Desjardin DE, Kropp BR, Norvell LL, Parker A, Vellinga EC, Vilgalys R, Hibbett DS. 2006. Major clades of Agaricales: a multi-locus phylogenetic overview. Mycologia 98: 982–995. http://dx.doi.org/10.3852/mycologia.98.6.982

Miyauchi S. 1998. Comparison Clitocybe acromelalga with Clitocybe sp. collected in France. Rapport of the Nagaoka University of Technology, sect. Bio-Ingeneering Kamitomioka 1603-1, Nagaoka, Japan.

Moncalvo J-M., Vilgalys R, Redhead SA, Johnson JE, James TY, Aime MC, Hofstetter V, Verduin SJW, Larsson E, Baroni TJ, Thorn RG, Jacobsson S, Clémençon H, Miller OK Jr. 2002. One hundred and seventeen clades of euagarics. Mol. Phylogenet. Evol. 23: 357–400. http://dx.doi.org/10.1016/S1055-7903(02)00027-1

Moreau PA, Courtecuisse R, Guez D, Garcin R, Neville P, Saviuc P, Seigle-Murandi F. 2001. Étude taxinomique d’une espèce toxique: Clitocybe amoenolens Malençon. Cryptogam. Mycol. 22: 95–117. http://dx.doi.org/10.1016/S0181-1584(01)80003-8

Nakamura K, Shoyama F, Toyama J, Tateishi K. 1987. Empoisonnement par le Dokou-sassa-ko ( Clitocybe acromelalga ). Jpn. J. Toxicol. 1: 35–39.

Nilsson RH, Kristiansson E, Ryberg M, Hallenberg N, Larsson K-H. 2008. Intraspecific ITS variability in the Kingdom Fungi as expressed in the International Sequence Databases and its implications for molecular species identification. Evol. Bioinf.4: 193–201.

Poumarat S, Neville P. 1993. Espèce de la zone du Quercus ilex au Maroc, montagnarde en France, Clitocybe amoenolens Malençon. Bull. Fédér. Assoc. Mycol. Médit. 4: 16–19.

Redhead SA, Lutzoni F, Moncalvo JM, Vilgalys R. 2002. Phylogeny of agarics: partial systematics solutions for core omphalinoid genera in the Agaricales (Euagarics). Mycotaxon 83: 19–57.

Romagnesi H. 1989. Curiosité mycologique: un champignon tortionnaire japonais: Clitocybe acromelalga Ichimura. Bull. Soc. Mycol. France 105: 131–132.

Raithelhuber J. 1995. Trichterlinge mitteleuropas. Metrodiana22(2): 52–94.

Raithelhuber J. 2004. Mitteleuropäische Trichterlinge. Gattungen Clitocybe, Pseudolyophyllum und Paralepista. JH. Raithelhuber Außenseiterverlag, Stuttgart.Rambaut A, Drummond AJ. 2007. Tracer v1.4. [Available from http://beast.bio.ed.ac.uk/Tracer].

Ridgway R. 1912. Color standards and color nomenclature. Washington, D.C., published privately (by the author). 43 p. + 53 color pls.

Saviuc PF, Danel VC, Moreau PA, Guez DR, Claustre AM, Carpentier PH, Mallaret MP, Ducluzeau R. 2001. Erythromelalgia and mushroom poisoning.Clin. Toxicol. 39: 403–407. http://dx.doi.org/10.1081/CLT-100105162

Saviuc PF, Danel VC, Moreau PA, Claustre AM, Ducluzeau R, Carpentier PH. 2002. Érythermalgie soudaine: cherchez le champignon! Rev. Méd. Interne 23: 394–399. http://dx.doi.org/10.1016/S0248-8663(02)00576-3

Saviuc PF, Danel VC. 2006. New syndromes in mushroom poisoning. Toxicol. Rev. 25: 199–209. http://dx.doi.org/10.2165/00139709-200625030-00004Singer R. 1986. The Agaricales in modern taxonomy, 4th ed. Koeltz Scientific Books, Koenigstein.


Paralepistopsis gen, nov. and Paralepista... 267

Stamatakis A. 2006. RAxML-VI-HPC: Maximum Likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22: 2688–2690. http://dx.doi.org/10.1093/bioinformatics/btl446

Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28: 2731–2739. http://dx.doi.org/10.1093/molbev/msr121

Thiers B. 2011. (continuously updated). Index Herbariorum: a global directory of public herbaria and associated staff. New York Botanical Garden’s Virtual Herbarium. http://sweetgum.nybg.org/ih/

Vilgalys R, Hester M. 1990. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J. Bacteriol. 172: 4238–4246.

Vizzini A. 2009. Due nuove combinazioni nel genere Cleistocybe (Basidiomycota, Agaricomycetes), con validazione di Hygrophorus pleurotoides. Micol. Veget. Medit. 24: 95–98.

Vizzini A, Musumeci E, Murat C. 2010a. Trichocybe, a new genus for Clitocybe puberula (Agaricomycetes, Agaricales). 2010. Fung. Diver. 42: 97–105. http://dx.doi.org/10.1007/s13225-010-0030-8

Vizzini A, Contu, M., Musumeci, E. and Ercole E. 2010b. A new taxon in the Infundibulicybe gibba complex (Basidiomycota, Agaricales, Tricholomataceae) from Sardinia (Italy). Mycologia 103: 203–208. http://dx.doi.org/10.3852/10-137

Vizzini A, Contu M., Ercole E. 2011. Musumecia gen. nov. in the Tricholomatoid clade (Basidiomycota, Agaricales) related to Pseudoclitocybe. Nord.J.Bot., Article first published online: 20 OCT 2011. http://dx.doi.org/10.1111/j.1756-1051.2011.01169.x

White TJ, Bruns TD, Lee S, Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. 315–322, in: MA Innis et al. (eds). PCR Protocols. Academic Press, London.


 

in Bon 1983 (DM XIII 51 :14) Section Gilvoideae (Harm.)Big : Spores non lacrymoïdes, odeur faible à farineuse ou fruitée. Pigment mixte ou peu incrustant.

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